The researchers began the work in the energy-producing portion of a cell, the mitochondria. In plants, aberrations within the mitochondrial genome can be associated with the inability to make pollen, a trait known as cytoplasmic male sterility that has been successfully exploited for the production of high-yielding hybrid seeds in many important crops. Naturally occurring CMS-based systems that are robust enough to facilitate commercial scale hybrid seed production are limited, however.
Normal tobacco seeds are dense and germinate; seeds from plants with the mitochondrial gene deletion are mostly hollow and don't germinate. Credit: Ralph Dewey, NC State University. "That's because the promoter that we chose not only failed to express during pollen formation, but also during early seed development," Dewey said.
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